References:
D. Marion, P.C. Driscoll, L.E. Kay, P.T. Wingfield, A. Bax, A.M. Gronenborn and G.M. Clore (1989) Biochemistry 28 6150-6156. (Link to Article)
D. Marion, L.E. Kay, S.W. Sparks, D.A. Torchia and A. Bax (1989) J. Am. Chem. Soc. 111 1515-1517. (Link to Article)
E.R.P. Zuiderweg and S.W. Fesik (1989) Biochemistry 28 2387-2391. (Link to Article)

Minimum labelling: ¹³C

Dimensions: 3

To start with, magnetisation is exchanged between all hydrogens using the NOE. Then the magnetisation is transferred to neighbouring ¹³C nuclei and back to ¹H for detection. Transfer either occurs to/from the aliphatic ¹³C nuclei or to/from the aromatic ¹³C nuclei (but not both) depending on the ¹³C frequency used during the pulse sequence.

This spectrum is used to obtain restraints for structure calculations. It will usually be recorded with 100% D₂O as the solvent – this minimises artefacts. This can be centred either on the aliphatic or on the aromatic carbons. The spectrum centred on the aromatic carbons can also be very useful for the assignment of the aromatic residues.