Protein NMR

A Practical Guide

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  • Introduction
  • Solution NMR
    • Assignment Theory
      • Visualising 3D Spectra
      • Triple Resonance Backbone Assignment
      • Triple Resonance Side-chain Assignment
      • Double Resonance Backbone Assignment
      • Double Resonance Side-chain Assignment
    • Assignment Practice
      • Initialising the HSQC
      • Peakpicking the 3D Spectra
      • Triple Resonance Backbone Assignment
        • Manual Assignment
        • Semi-automated Assignment
        • Sequence-Specific Assignment
        • Using HNCA/HNCO Spectra
      • Double Resonance Backbone Assignment
        • Neighbouring Residues
        • Directionality
        • Matching Peaks
        • Lining Up Strips
        • Sequence-Specific Assignment
      • Hints and Tips
    • Spectrum Descriptions
      • 1H-15N HSQC
      • HNCO
      • HN(CA)CO
      • HNCA
      • HN(CO)CA
      • CBCA(CO)NH / HN(CO)CACB
      • CBCANH / HNCACB
      • CC(CO)NH
      • H(CCO)NH
      • HBHA(CO)NH
      • HCCH-TOCSY
      • HCCH-COSY
      • 15N-TOCSY-HSQC
      • 13C-HMQC
      • H-H NOESY
      • 15N-NOESY-HSQC
      • 13C-NOESY-HSQC
      • 13C-HMQC-NOESY
  • Solid-State MAS NMR
    • Solid-state Assignment Tutorial
    • CCPNmr Analysis Solid-state Tips
    • Spectrum Descriptions
      • PDSD
      • DARR
      • NCA
      • NCO
      • NCACX
      • NCOCX
      • NCACB
      • CANCO
      • CANCOCX
      • CHHC
      • NHHC
  • General NMR
    • Chemical Shifts
      • Basics
      • Chemical Shift Anisotropy
      • Chemical Exchange
      • Chemical Shift Mapping
      • Random Coil Chemical Shifts
      • Secondary Structure
      • Structure Calculation
      • Chemical Shift Prediction
    • Isotopic Labelling
      • 15N
      • 15N,13C
      • 15N,13C,2H
      • IVL side-chain methyl groups
      • SAIL (Stereo-Array Isotope Labelling)
      • 1,3-13C- and 2-13C-Glycerol
      • Amino Acid Specific
      • Cell-free
      • Segmental
      • Other Labelling Schemes
    • Literature
    • Software
      • Processing
      • Assignment
      • Automatic Assignment
      • Structure Calculation
      • Molecular Viewers
      • Validation
      • Chemical Shift Analysis
      • RDC Analysis
      • Analysis of Dynamics Data
      • Pulse Sequence Simulation
      • Lab Software Pages
    • NMR Links
    • Glossary / Abbreviations
  • CCPNmr Analysis
    • Version 1.0 Basics
      • New Project
      • Molecules
      • Reading in Spectra
      • Windows and Axes
      • Spectrum Attributes
      • Navigation
      • Mouse Usage
      • Marks and Rulers
      • Peakpicking
      • Resonances and Spin Systems
      • Assignment of Peaks
      • Peak Lists
      • Strips
      • Tables
      • Keyboard Shortcuts
    • Version 2.x Basics
      • New Project
      • Molecules
      • Reading in Spectra
      • Windows and Axes
      • Spectrum Attributes
      • Navigation
      • Mouse Usage
      • Marks and Rulers
      • Peakpicking
      • Resonances and Spin Systems
      • Assignment of Peaks
      • Peak Lists
      • Strips
      • Tables
      • Keyboard Shortcuts
    • CCPNmr Analysis Solid-state Tips
      • Experiment Types
      • Overlaying Spectra
      • Double Cross-hair Mouse
      • Organising Windows and Spectra
      • Strips and Separators
      • Making Strip Plots
      • Tolerances
      • 1D Spectra
      • Assignments
      • Tentative Assignments
      • Identifying Side-band Peaks
      • Marking Side-band Peaks
      • Double-Quantum Axes
      • Labelling Schemes
      • Editing Labelling Schemes
      • Distance Restraints
    • Figures with CCPNmr Analysis
  • Contact

General NMR

This section contains some general information of interest to Protein NMR spectroscopists, including chemical shifts, isotopic labelling strategies, software, literature and NMR related links.

Content

  • Introduction
  • Solution NMR
  • Solid-State MAS NMR
  • General NMR
    • Chemical Shifts
    • Isotopic Labelling
    • Literature
    • Software
    • NMR Links
    • Glossary / Abbreviations
  • CCPNmr Analysis
  • Contact
by Victoria A. Higman, last modified on 31 October 2012