Reference:
A. Lange, S. Luca and M. Baldus (2002) J. Am. Chem. Soc. 124 9704-9705. (Link to Article)

Minimum labelling: ¹⁵N, ¹³C

Dimensions: 2

Magnetisation is transferred from ¹H to ¹³C via cross polarisation. In three sucessive steps it is first transferred back to ¹H, then to other ¹H nuclei nearby, and finally back to ¹³C for detection. The chemical shift is evolved on the ¹³C nuclei following the initial cross polarisation and detected on the ¹³C nuclei at the end, resulting in a 2D carbon-carbon spectrum, but which encodes information about proton-proton distances of the protons attached to the carbon atoms.

This experiment is particularly useful for obtaining proton-proton structural restraints which can be used in protein structure calculations.