A. Bax, G.M. Clore, P.C. Driscoll, A.M.Gronenborn, M. Ikura and L.E. Kay (1990) J. Magn. Reson. 87 620-627. (Link to Article)
L.E. Kay, M. Ikura and A. Bax (1990) J. Am. Chem. Soc. 112 888-889. (Link to Article)
M. Ikura, L.E. Kay and A. Bax (1991) J. Biomol. NMR 1 299-304. (Link to Article)

Minimum labelling: 15N, 13C

Dimensions: 3

Magnetisation is transferred from the side-chain hydrogen nuclei to their attached 13C nuclei. Magnetisation is then exchanged between neighbouring 13C nuclei via the J-coupling and finally transferred back to the side-chain hydrogen atoms for detection.

HCCH-COSY magnetisation transfer

This spectrum can be useful in aiding side-chain assignment.

HCCH-COSY spectrum